TY - JOUR AU - Lakshmana Rao, A. AU - Raja, T. AU - Harika, B. Madhu PY - 2019/03/05 Y2 - 2024/03/29 TI - DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF PARACETAMOL AND LORNOXICAM IN BULK AND PHARMACEUTICAL DOSAGE FORM JF - International Journal of Research in AYUSH and Pharmaceutical Sciences JA - IJRAPS VL - 2 IS - 8 SE - Articles DO - UR - https://ijraps.in/index.php/ijraps/article/view/55 SP - 275-281 AB - A simple, rapid, accurate and precise isocratic reversed phase high performance liquid chromatographic method has been developed and validated for simultaneous estimation of Paracetamol and Lornoxicam in tablet dosage form. The chromatographic separation was carried out on Zorbax C18 column (150 mm x 4.6 mm I.D., 5 µm particle size) with a mixture of 20 mM ammonium acetate pH 3.2 buffer and acetonitrile in the ratio of 60:40 v/v as a mobile phase at a flow rate of 1.0 mL/min. UV detection was performed at 265 nm. The retention times were 2.74 minutes and 5.36 minutes for Paracetamol and Lornoxicam respectively. Calibration plots were linear (r2=0.999 for both Paracetamol and Lornoxicam respectively) over the concentration range of 6.25-250 µg/mL for Paracetamol and 0.1-4 µg/mL for Lornoxicam. The method was validated for linearity, precision, accuracy, ruggedness and robustness. The proposed method was successfully used for simultaneous estimation of Paracetamol and Lornoxicam in tablet dosage form. Validation studies revealed that the proposed method is specific, rapid, reliable and reproducible. The high % recovery and low % RSD confirms the suitability of the proposed method for routine quality control analysis of Paracetamol and Lornoxicam in bulk and tablet dosage form. ER -