IN VITRO ANTI OXIDANT ACTIVITY OF CHROMATOGRAPHICALLY SEPARATED FRACTIONS FROM THE LEAVES OF AGERATUM CONYZOIDES L

Prasanta Kumar Mitra; Tanaya Ghosh; Prasenjit Mitra

Prasanta Kumar Mitra Professor & Head, Department of Medical Biotechnology, Sikkim Manipal University, Sikkim, Manipal Institute of Medical Sciences, Gangtok, Sikkim
Tanaya Ghosh Professor & Head, Department of Medical Biotechnology, Sikkim Manipal University, Sikkim, Manipal Institute of Medical Sciences, Gangtok, Sikkim
Prasenjit Mitra Department of Biochemistry, All India Institute of Medical Sciences, Jodhpur, Rajasthan

Keywords: Ageratum conyzoides L. leaves, chromatographic separation, anti oxidant activity, xanthine-xanthine oxidase assay, linoleicacid peroxidation assay, DPPH photometric assay.

Abstract

Synthetic anti oxidants are not safe for human health. It is often claimed that they may develop carcinoma in human body. Therefore, search for natural anti oxidants was going on and extended up to plant sources. Many medicinal plants are known having anti oxidant activity. Aageratum conyzoides Linn. is one such plant. In order to isolate anti oxidant compound (s) from the leaves of A. conyzoides L. the present study was undertaken. In isolation study silica gel G column chromatography of the powdered leaves of A.conyzoides L. was done when six fractions were separated. In vitro anti oxidant activity of these six fractions was measured by superoxide anion generation with help of xanthine-xanthine oxidase assay and with linoleic acid peroxidation assay as well as DPPH photometric assay. Results showed that fourth fraction had maximum anti oxidant activity. Inhibitory activities of xanthine oxidation, linoleic acid peroxidation and scavenging capacity of DPPH by the fourth fraction were respectively 96%, 97% and 96% whereas for other five fractions inhibitory activities were quite low.

Anti oxidant activity is known to be associated with compounds like total phenol, flavonoids, ascorbic acid and carotenoids. These compounds were estimated in the separated six fractions after chromatography of powdered leaves of A. conyzoides L. Results showed that fourth fraction had total phenol, flavonoids, ascorbic acid and carotenoids in the concentrations of 58 mg/mg dry wt, 88 mg/mg dry wt, 22 mg/g dry wt and 25 mg/g dry wt respectively. The amounts were significantly higher in comparison to that of other fractions. In vitro anti oxidant activity of the fourth fraction was, therefore, related with high amounts of total phenol, flavonoids, ascorbic acid and carotenoids. Present study indicated that the separated fourth fraction after silica gel G column chromatography of powdered leaves of A.conyzoides L. may be used as natural anti oxidant.

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