Method Development and Validation of Lorazepam by Using RP-HPLC in Pharmaceutical Formulation

Authors

  • J.R.V.Prasanna
  • D.Narendra
  • K.Susritha Reddy
  • A.Lakshmi Tulasi
  • A.Nikhitha
  • M.Krishna Sowmya Sri VJ’s College of Pharmacy, Diwancheruvu, Rajamahendravaram, Andhra Pradesh, India.

DOI:

https://doi.org/10.47070/ijraps.v7i4.139

Abstract

High pressure liquid chromatography (RP-HPLC) is a column chromatographic technique employing high pressure pump to pass both mobile phase and sample mixture through stationary phase column and perform efficient separation. A C-18 column with mobile phase containing Acetonitrile: methanol (80: 20) was used. The flow rate was 1.0ml/min. and were monitored at 210 nm. The retention time for Lorazepam was 1.3 min. The method was validated for linearity, accuracy, precision, limit of detection. The Mean percent recovery of Lorazepam from tablet formulation was found to be 94.80 %.

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Published

22-04-2023

How to Cite

1.
J.R.V.Prasanna, D.Narendra, K.Susritha Reddy, A.Lakshmi Tulasi, A.Nikhitha, M.Krishna Sowmya Sri. Method Development and Validation of Lorazepam by Using RP-HPLC in Pharmaceutical Formulation. IJRAPS [Internet]. 2023 Apr. 22 [cited 2025 Aug. 2];7(4):1-3. Available from: https://ijraps.in/index.php/ijraps/article/view/139

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Section

Articles