Simultaneous Estimation of Metformin and Teneligliptin in Pharmaceutical Formulation by Using UV Spectroscopy

Abstract

Three new UV spectrophotometric methods namely simultaneous equation, absorbance ratio and dual wavelength methods were developed and validated for simultaneous estimation of Metformin and Teneligliptin in bulk drug and tablet formulation which were simple, rapid, sensitive, precise and accurate. In simultaneous equation method, absorbance was measured at 233nm for Metformin and 241nm for Teneligliptin. In absorbance ratio method, absorbance was measured at 244nm for Metformin and 233nm for Teneligliptin. In dual wavelength method, two wavelengths were selected for each drug, the absorbances was measured at 225 and 251nm for Metformin and 240 and 221nm for Teneligliptin. Developed methods were validated according to ICH guidelines including parameters viz., specificity, linearity and range, precision, accuracy, limit of detection and limit of quantification. All the three methods showed linear response in the concentration range of 2-10µg/mL for Metformin and 0.5-2.5µg/mL for Teneligliptin with a low correlation coefficient. Results of method validation parameters follows ICH guideline acceptable limits. Methods were found to be simple, rapid, sensitive, economical and hence can be useful for simultaneous estimation of Metformin and Teneligliptin in pure drug and commercial tablet formulation for routine quality control analysis.